The definition of magnetic beads

Magnetic beads are a kind of superparamagnetic nanoparticles, the general particle size between tens of nanometers to more than one hundred microns, different particle sizes have different uses in the field of protein purification, cell sorting, nucleic acid extraction and chemiluminescence, these different uses are not only related to the particle size of magnetic beads, but also have a close relationship with the chemical/biological modification of the surface of magnetic beads.

Preparation of magnetic beads

In the process of preparation of magnetic beads, agglomeration is easy to occur due to the effect of van der Waals force between particles, but because of its adsorbability, charged ions are adsorbed on the surface to form a double electric layer structure with moving potential gradient to prevent agglomeration. In order to solve this problem, magnetic beads are often dispersed evenly in the solution to form colloids, and agglomeration between particles can be avoided to a certain extent due to Brownian motion.

ATS high pressure homogenizer disperses magnetic bead solution

Step 1: Appearance

After the ATS high pressure homogenizer, the magnetic beads are uniformly dispersed in the solution

                                      (Left before dispersion, right after dispersion)

2. Particle size detection

The particle size of D90 was 610.7nm after dispersing by high pressure homogenizer